|
|
Iranian Biomedical Journal، جلد ۸، شماره ۱، صفحات ۱۳-۱۸
|
|
|
| عنوان فارسی |
پروتئین نوترکیب ریبوزومی (L۷/L۱۲) از باکتری بروسلا آبورتوس و واکنش آن با سرم افراد مبتلا به بروسلاز |
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
High Level Expression of Recombinant Ribosomal Protein (L7/L12) from Brucella abortus and Its Reaction with Infected Human Sera |
|
| چکیده انگلیسی مقاله |
Brucellosis, caused by Brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. Various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. Among Brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. In this study, the immunogenic ribosomal protein L7/L12 gene from Brucella abortus, S19, was amplified by PCR and sub-cloned to prokaryotic expression vector pET28a. Escherichia coli BL21 (DE3) pLysS was transformed with pET28a-L7/L12 and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography with Ni-NTA resin. The concentration of purified recombinant protein calculated to 8 mg/L of initial culture. The integrity of product was confirmed by Western-blot analysis using a standard rabbit anti Brucella abortus ribosomal protein L7/L12 antibody. Sera reactivity of five infected individual were further analyzed against the recombinant ribosomal L7/L12 protein. Data indicated that recombinant ribosomal L7/L12 protein from Brucella abortus was recognized by patient sera |
|
| کلیدواژه‌های انگلیسی مقاله |
|
|
| نویسندگان مقاله |
حمید ابطحی | hamid abtahi
علی هاتف سلمانیان | ali hatef salmanian
سیما رافتی | sima rafati
قربان بهزادیان نژاد | ghorban behzadian nejad
زهیر محمد حسن | zuhaiar mohammad hassan
|
|
| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-240&slc_lang=en&sid=en |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
مقاله کامل |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|