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Iranian Biomedical Journal، جلد ۱۴، شماره ۱، صفحات ۲۳-۳۲
عنوان فارسی Cytotoxicity of Subtoxic AgNP in Human Hepatoma Cell Line (HepG۲) after Long-Term Exposure
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عنوان انگلیسی Cytotoxicity of Subtoxic AgNP in Human Hepatoma Cell Line (HepG2) after Long-Term Exposure
چکیده انگلیسی مقاله Background: We aimed at evaluating the toxicity effects of low (subtoxic) concentrations of silver nanoparticles (AgNP, 5-10 nm) in human hepatoblastoma (HepG2) cell line after and during a period of about one month. Methods: XTT and MTT assays were used to draw a dose-response curve IC50 (half maximal inhibitory concentration) value of the AgNP on HepG2 cells was calculated to be 2.75-3.0 mg/l. The cells were exposed to concentrations of 0% (control), 1%, 4% and 8% IC50 of AgNP (corresponding to 0.00, 0.03, 0.12 and 0.24 mg/l of AgNP, respectively) for four consecutive passages. The treated cells were compared to the control group with respect to morphology and proliferation at the end of the period. Results: The biochemical studies revealed significant increases of lactate dehydrogenase and alanine aminotransferase enzyme activity in the culture media of cells receiving 4% and 8% IC50 the increases in the aspartate aminotransferase enzyme activity and nitric oxide concentration became significant at 8% IC50. In the cell extracts, the average total protein and activity of glutathione peroxidase enzyme remained unchanged the decrease in the average content of glutathione (GSH) and superoxide dismutase (SOD) activity became significant at 4% and 8% IC50. There were increases in lipid peroxidation (significant at 4% and 8% IC50) and cytochrome c content (significant at 8% IC50). The accumulations of the effects, during the experiment from one generation to the next, were not statistically remarkable except in cases of GSH and SOD. The results indicate clearly the involvement of oxidative changes in the cells after exposure to low doses of AgNP. Conclusion: The results might help specify a safer amount of AgNP for use in different applications.
کلیدواژه‌های انگلیسی مقاله Cytotoxicity, Liver, Enzyme activity
نویسندگان مقاله آذین نوروزی | azin nowrouzi


خدیجه مقرازی | khadijeh meghrazi


تقی گل محمدی | taghi golmohammadi


ابوالفضل گلستانی | abolfazl golestani


شهین احمدیان | shahin ahmadian


مهشید شفیع زاده | mahshid shafiezadeh


زهرا شجری | zahra shajary


شهناز خاقانی | shahnaz khaghani


آذیتا ان امیری | azita n amiri


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-167&slc_lang=en&sid=en
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