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Iranian Biomedical Journal، جلد ۱۵، شماره ۳، صفحات ۶۶-۷۲
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| عنوان فارسی |
بررس نیمه کمی بیان hoxA-۱۱, lif and bteB۱ mRNAs در بیماران مبتلا به اندومتریوز در مرحله ترشحی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Semi-Quantitative Analysis of HOXA11, Leukemia Inhibitory Factor and Basic Transcriptional Element Binding Protein 1 mRNA Expression in the Mid-Secretory Endometrium of patients with endometriosis |
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| چکیده انگلیسی مقاله |
Background: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory. Methods: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR. Results: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to ß-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P< 0.05). A similar trend was seen in BTEB1 mRNA expression. Conclusion: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis. |
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| نویسندگان مقاله |
زهره علیزاده | zohreh alizadeh
ناصر شکرزاده | naser shokrzadeh
مسعود سعیدی جم | massoud saidijam
مرضیه فریمانی سنویی | marzieh farimani sanoee
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| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-146&slc_lang=en&sid=en |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Related Fields |
| نوع مقاله منتشر شده |
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