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Iranian Biomedical Journal، جلد ۱۷، شماره ۱، صفحات ۳۶-۴۱
عنوان فارسی نقش ایمونوهیستوشیمی و تولوئیدین بلو برای شناسائی هلیکوباکترپیلوری در نمونه های بیوپسی معدی بیماران مبتلا به گاستریت
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عنوان انگلیسی The Immunohistochemistry and Toluidine Blue Roles for Helicobacter pylori Detection in Patients with Gastritis
چکیده انگلیسی مقاله Background: Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily available in laboratories. Therefore, this study was conducted to compare hematoxylin and eosin (H&E), Giemsa and toluidine blue staining with immunehistochemistry for detection of H. pylori in patients with gastritis and also to correlate the results of these staining methods with pathological grading. Methods: We reviewed 54 consecutive gastric biopsy specimens stained by H&E and Giemsa as well as by toluidine blue and immunohistochemistry stains for H. pylori. Results: H. pylori was positively identified by IHC in 43 (79.63%) patients, while positive samples were found in 18 (33.33%), 24 (44.44%) and 33 (61.11%) patients using H&E, Giemsa and toluidine blue staining methods. Our results showed that classical histological staining methods are not sensitive enough to identify low numbers or coccoid forms of organism, while toluidine blue and immunohistochemistry play an important role in detection of H. pylori infection. Conclusion: Toluidine blue has been proved to be much more reliable than H&E and Giemsa in detection of H. pylori. In addition, in post treatment biopsies and in biopsies with unexplained chronic active gastritis without histological evidence of H. pylori should have immunohistochemistry done to detect possible low density or coccoid form of organisms.
کلیدواژه‌های انگلیسی مقاله Helicobacter pylori, Immunohistochemistry, Gastritis
نویسندگان مقاله راضیه تجلی | raziye tajalli


ملیحه نوبخت | maliheh nobakht


هاجر محمدی برزلیقی | hajar mohammadi barzelighi


شهرام آگاه | shahram agah


عبدالعزیز رستگار لاری | abdolaziz rastegar lari


علیرضا صادقی پور | alireza sadeghipour


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-379&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
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