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Iranian Biomedical Journal، جلد ۱۷، شماره ۲، صفحات ۵۴-۶۱
عنوان فارسی بررسی بیان ایزوفرم های پروتیینی ژنChromosome (RBMY) RNA-Binding Motif Gene on Y در بافت بیضه و لاین سلولی سرطانی مشتق از سلول های زایا (NT۲)
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression Analysis of RNA-Binding Motif Gene on Y Chromosome (RBMY) Protein Isoforms in Testis Tissue and a Testicular Germ Cell Cancer-Derived Cell Line (NT2)
چکیده انگلیسی مقاله a key factor in spermatogenesis and disorders associated with this protein have been recognized to be related to male infertility. Although it was suggested that this protein could have different functions during germ cell development, no studies have been conducted to uncover the mechanism of this potential function yet. Here, we analyzed the expression pattern of RBMY protein isoforms in testis compared to NT2, a testicular germ cell cancer-derived cell line, to test probability of differential expression of RBMY protein isoforms at different spermatogenesis stages. Methods: Full length and a segment of RBMY gene were cloned and expressed in E. coli. Anti-human RBMY antibody was produced in rabbit using the recombinant proteins as antigen. Western-blot and immunofluorescence were conducted for detection and comparison of RBMY protein isoforms. Results: Selected segment of RBMY protein resulted in producing a mono-specific antibody. As results shows, only the longest isoform of RBMY was expressed at protein level in NT2 cell line, while three isoforms of this protein were detected in the whole testis lysate. Conclusion: The results imply that different alternative splicing may happen in testis cells and probably difference of RBMY function during spermatogenesis is due to the differential expression of RBMY protein isoforms. These results and further experiments on RBMY isoforms can help to obtain a better understanding of the function of this protein, which may increase our knowledge about spermatogenesis and causes of male infertility.
کلیدواژه‌های انگلیسی مقاله Protein isoforms, Spermatogenesis, Male infertility
نویسندگان مقاله مهدی علیخانی | mehdi alikhani


مهدی شریفی تبار | mehdi sharifi tabar


شهاب میرشاه ولدی | shahab mirshahvaladi


ابوالفضل خیمه | abolfazl kheimeh


محمدعلی صدیقی گیلانی | mohammad ali sadighi gilani


مرجان صباقیان | marjan sabbaghian


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-472&slc_lang=fa&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
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