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Iranian Biomedical Journal، جلد ۱۷، شماره ۴، صفحات ۱۷۹-۱۸۶
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| عنوان فارسی |
انجماد شیشه ای و ذوب بافت تخمدان انسانی تاثیر اندکی بر بیان ژنهای وابسته به آپوپتوز دارد |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Human Ovarian Tissue Vitrification/Warming Has Minor Effect on the Expression of Apoptosis-Related Genes |
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| چکیده انگلیسی مقاله |
Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified and warmed and the other samples were considered as control. Apoptosis was assessed by a transmission electron microscope and also by molecular analysis of pro-apoptotic (Fas, FasL, Bax, p53, caspase8, and caspase3) and antiapoptotic (Bcl-2 and BIRC5) genem RNA levels using real-time RT-PCR before and after vitrification. Results: No sign of apoptosis was shown ultrastructurally in vitrified samples. The level of FasL, Bcl-2, Bax, p53, and caspase3 mRNA and Bax:Bcl-2 ratio were similar in non-vitrified and vitrified groups however, the expression of Fas and caspase8 genes was higher and BIRC5 was lower in vitrified samples compared to non-vitrified group (P< 0.05). Conclusion: The fine structure of human vitrified ovarian tissue was well preserved moreover, vitrification was shown to affect the expression of some apoptosis-related genes. However, additional study is needed to confirm this observation. |
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| کلیدواژه‌های انگلیسی مقاله |
Vitrification, Apoptosis, Gene expression, Ovary, Humans |
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| نویسندگان مقاله |
معصومه عبدالهی | maasoume abdollahi
مژده صالح نیا | mojdeh salehnia
ساغر صالح پور | saghar salehpour
نسیم قربانمهر | nassim ghorbanmehr
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| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-485&slc_lang=en&sid=en |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Related Fields |
| نوع مقاله منتشر شده |
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