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Iranian Biomedical Journal، جلد ۱۹، شماره ۴، صفحات ۲۲۰-۲۲۵
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| عنوان فارسی |
Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis |
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| چکیده انگلیسی مقاله |
Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF-9,, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results: Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion: Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes. |
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| کلیدواژه‌های انگلیسی مقاله |
Vitrification, Gene expression, Humans |
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| نویسندگان مقاله |
زهرا شمس mofarahe | zahra shams mofarahe
معرفت غفاری نوین | marefat ghaffari novin
مینا جعفرآبادی | mina jafarabadi
مژده صالح نیا | mojdeh salehnia
محسن نوروزیان | mohsen noroozian
نسیم قربانمهر | nassim ghorbanmehr
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| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-563&slc_lang=en&sid=en |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Related Fields |
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