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Iranian Biomedical Journal، جلد ۲۱، شماره ۱، صفحات ۴۸-۵۶
عنوان فارسی Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production
چکیده فارسی مقاله
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عنوان انگلیسی Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production
چکیده انگلیسی مقاله Background: Alginate is a linear polysaccharide consisting of guluronate (polyG) and mannuronate (polyM) subunits. Methods: In the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. The optimization of cell growth and alginate lyase (algL) production was carried out by the addition of 0.8% alginate and 0.2-0.3 M NaCl to the culture medium. Of 10 isolates, one was selected based on its fast growth rate on minimal 9 medium containing 0.4% sodium alginate. The selected bacterium, identified based on morphological and biochemical characteristics as well as 16S rDNA sequence data, was confirmed to be an isolate belonging to the genus Bacillus and designated as Bacillus sp. TAG8. Resuls: The results showed the ability of Bacillus sp. TAG8 to utilize alginate as a sole carbon source. Bacillus sp. TAG8 growth and algL production were augmented with an increase in sodium alginate concentration and also by the addition of 0.2-0.3 M NaCl. Molecular analysis of TAG8 algL gene showed 99% sequence identity with algL of Pseudomonas aeruginosa PAO1. algL produced by Bacillus sp. TAG8 cleaved both polyM and polyG blocks in alginate molecule as well as acetylated alginate residues, confirming the bifunctionality of the isolated lyase. Conclusion: The identification of novel algL genes from microbial communities constitutes a new approach for exploring lyases with specific activity against bacterial alginates and may thus contribute to the eradication of persistent biofilms from clinical samples.
کلیدواژه‌های انگلیسی مقاله Alginate, Bacillus spp., Alginate lyase, Pseudomonas biofilm
نویسندگان مقاله حدیث طوافی | hadis tavafi
department of microbiology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

احیا عبدی علی | ahya abdi ali
department of microbiology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

پریناز قدم | parinaz ghadam
department of biotechnology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

سارا غروی | sara gharavi
department of biotechnology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-621&slc_lang=en&sid=en
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