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Iranian Biomedical Journal، جلد ۲۱، شماره ۱، صفحات ۱۶-۲۳
عنوان فارسی Methylation Status of H۱۹/IGF۲ Differentially Methylated Region in in vitro Human Blastocysts Donated by Healthy Couples
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عنوان انگلیسی Methylation Status of H19/IGF2 Differentially Methylated Region in in vitro Human Blastocysts Donated by Healthy Couples
چکیده انگلیسی مقاله Backgrund: Imprinted genes are a unique subset of few genes, which have been differentially methylated region (DMR) in a parental origin-dependent manner during gametogenesis, and these genes are highly protected during pre-implantation epigenetic reprogramming. Several studies heve shown that the particular vulnerability of imprinting genes during suboptimal pre- and peri-conception microenvironments often occur by assisted reproduction techniques (ART). This study investigated the methylation status of H19/IGF2 DMR at high-quality expanding/expanded human blastocysts donated by healthy individuals to evaluate the risks linked to ART. Method: Methylation levels of H19/IGF2 DMR were analyzed by bisulfite conversion and sequencing at 18 CpG sites (CpGs) located in this region. Result: Results showed that the overall percentage of methylated CpGs and the proportion of hyper-methylated clones of H19/IGF2 DMR in analyzed blastocysts were 37.85±4.87% and 43.75±5.1%, respectively. For validation of our technique, the corresponding methylation levels of peripheral human lymphocytes were defined (49.52±1.86% and 50%, respectively). Conclusion: Considering the absence of in vivo produced human embryos, it is not possible to conclude that the methylation found in H19/IGF2 DMR is actually normal or abnormal. Regarding the possible risks associated with ART, the procedures should be optimized in order to at least reduce some of the epigenetic risks.
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نویسندگان مقاله مرضیه درخشان هوره | marzieh derakhshan horeh
department of anatomy, school of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فرید ابوالحسنی | farid abolhassani
department of anatomy, school of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فرنوش جعفرپور | farnoosh jafarpour
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, academic center for education, culture and research acecr , isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

اشرف معینی | ashraf moini
department of endocrinology and female infertility, reproductive biomedicine research centre, royan institute for reproductive biomedicine, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

خدیجه کربلایی | khadijeh karbalaie
department of cellular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سید مرتضی حسینی | sayyed morteza hosseini
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, academic center for education, culture and research acecr , isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سمیه ostadhosseini | somayyeh ostadhosseini
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, academic center for education, culture and research acecr , isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, academic center for education, culture and research acecr , isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-624&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Medical Biotechnology
نوع مقاله منتشر شده مقاله کامل
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