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Journal of Dentistry, Shiraz University of Medical Sciences، جلد ۱۹، شماره ۲، صفحات ۱۲۴-۰
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عنوان انگلیسی Comparison of Osteogenic and Chondrogenic Differentiation Ability of Buccal Fat Pad Derived Mesenchymal Stem Cells and Gingival Derived Cells
چکیده انگلیسی مقاله Statement of the Problem: One major goal of tissue engineering and regenerative medicine is to find an appropriate source of mesenchymal stem cells (MSCs) with higher differentiation ability. Purpose: In this experimental study, the osteogenic and chondrogenic differentiation ability of buccal fat pad derived MSCs (BFP-MSCs) with gingival derived cells (GDCs) were compared. Materials and Method: BFP-MSCs and GDCs were cultured enzymatically and expanded. The expanded cells were analyzed for membrane-associated markers, using flow cytometry. Then the ability of these cells to differentiate into osteocyte and chondrocyte was assessed morphologically and by mRNA expression of collagen I (COLL), BGLA and bone morphogenetic protein 2 (BMP2) using qRT-PCR. Results: Flow cytometry analysis showed that both BFP-MSCs and GDCs expressed the characteristic stem cell markers such as CD73, CD44, and CD90, whereas they did not express hematopoietic markers. Mineralized calcium deposition was observed apparently in BFP-MSCs cultured in osteogenic medium but GDCs showed fewer mineralized nodules. The mRNA expression levels of BGLA and BMP2 showed 7×10 5 and 733-fold more mRNA expression in BFP-MSCs treated with differentiation media compared to the control group. In chondrogenic differentiation, BFP-MSCs transformed from a spindle to a cuboidal shape while GDCs showed only a slight transformation. In addition, mRNA expression of COLL showed 282-fold higher expression in BFP-MSCs in comparison to the control group. Such significant difference in mRNA expression of BGLA, BMP2, and COLL was not observed in GDCs compared to their corresponding controls. Conclusion: Based on the present results, BFP yields a greater proportion of stem cells compared to gingiva. Therefore, this tissue can be introduced as an easily available source for the treatment of periodontal defects and other maxillofacial injuries.
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نویسندگان مقاله | Hamid Ghaderi
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.


| Mahboobeh Razmkhah
Oral and Dental, Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.


| Farin Kiany
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.


| Nooshafarin Chenari
Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.


| Mohammad Reza Haghshenas
Institute for Cancer Research, Dept. of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.


| Abbas Ghaderi


نشانی اینترنتی http://dentjods.sums.ac.ir/index.php/JDSUMS/article/view/1688
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/331/article-331-623445.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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