این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Agricultural Science and Technology، جلد ۷، شماره ۱، صفحات ۳۱-۴۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Antigenic Analysis of the Coat Protein of Alfalfa mosaic virus and its Involvement in Aphid Transmission
چکیده انگلیسی مقاله Two strains -425 and Y87/47- of Alfalfa mosaic virus (AMV) were propagated in and purified from Nicotiana tabacum cv. Samsun NN. Thirty-three AMV specific monoclonal antibodies (MAbs) from two fusions were raised against strain 425. These antibodies were of isotypes IgG1 and IgM. MAbs recognised three types of epitope. Group I did not react with the virus particle surface or viral coat protein of two strains in PTA-ELISA, but they reacted with a 30-kDa structural coat protein of AMV by immunoblot analysis only and were able to recognise cryptotopes. Group II reacted with metatopes of both strains in PTA-ELISA. Group III reacted with a 30-kDa structural coat protein of AMV by immunoblot analysis and in PTA-ELISA for the Y87/47 strain only. Immunoblocking experiments in which suspensions of purified AMV and MAb were offered between parafilm membranes for acquisition by Myzus persicae revealed that MAb-2 was effective in blocking (inhibiting) transmission. This result suggests that the epitope which was localised by MAb-2 plays a role in the aphid transmission of AMV.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://journals.modares.ac.ir/browse.php?a_code=A-23-1000-2475&slc_lang=en&sid=23
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/476/article-476-591553.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات