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Journal of Agricultural Science and Technology، جلد ۱۲، شماره ۴، صفحات ۴۵۹-۴۷۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran
چکیده انگلیسی مقاله To determine ancient seed species, 3250-3450 year-old charred seeds obtained from different Iranian archaeological sites were studied using Scanning Electron Microscopy (SEM) and molecular analysis. SEM analysis of ancient seeds revealed that the surface of the seeds was damaged. Therefore, we could not accurately identify their species. Molecular analysis on ancient specimens was done on different samples obtained from Masjede Kabood (Tabriz), Tepe Rahmat Abad (Pasargad) and Tepe Sagz Abad (Qazvin plain). The specific primer pairs were designed based on a part of the promoter region of the High Molecular Weight (HMW) glutenin gene and a short fragment of the vrs1 gene were verified on samples of modern wheat and barley varieties, respectively. The designated primers failed to amplify ancient DNAs (aDNAs) obtained from Masjede Kabood and Tepe Rahmat Abad, but successfully amplified the aDNA obtained from Tepe Sagz Abad. This finding was expected since the latter seeds had a better morphological preservation in comparison to the former ones. The accuracy of the amplified products was further proved by cloning and sequencing.
کلیدواژه‌های انگلیسی مقاله Wheat,Barley,Seed,aDNA,Ancient DNA,Molecular analyses
نویسندگان مقاله | H. Mahmoudi Nasab
Department of Genetics, Faculty of Basic Science, Tarbiat Modares University, P. O. Box: 14115-175, Tehran, Islamic Republic of Iran.


| M. Mardi
Department of Genomics, Agricultural Biotechnology Research Institute, Karaj, Islamic Republic of Iran.


| H. Talaee
Department of Archaeology, Faculty of Letters and Humanities, Tehran University, Tehran, Islamic Republic of Iran.


| H. Fazeli Nashli
Department of Archaeology, Faculty of Letters and Humanities, Tehran University, Tehran, Islamic Republic of Iran.


| S. M. Pirseyedi
Department of Genomics, Agricultural Biotechnology Research Institute, Karaj, Islamic Republic of Iran.


| A. Hejabri Noubari
Department of Archaeology, Faculty of Humanities, Tarbiat Modares University, Tehran, Islamic Republic of Iran.


Mowla | S. J. Mowla*
Department of Genetics, Faculty of Basic Science, Tarbiat Modares University, P. O. Box: 14115-175, Tehran, Islamic Republic of Iran.
نشانی اینترنتی http://journals.modares.ac.ir/browse.php?a_code=A-23-1000-4031&slc_lang=en&sid=23
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/476/article-476-591462.pdf
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