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Iranian Biomedical Journal، جلد ۲۲، شماره ۴، صفحات ۲۷۵-۲۸۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Preparation and Evaluation of Rifampicin and Co-trimoxazole-loaded Nanocarrier against Brucella melitensis Infection
چکیده انگلیسی مقاله Background: Brucellosis or Malta fever is a contagious infection common between human and domestic animals. Many antibiotics are used for brucellosis treatment, but they are not efficient and put heavy burden on society. Co-trimoxazole and rifampicin are two candidates for brucellosis treatment. In this study, we aimed to enhance the efficacy of these antibiotics using designed nanoparticles. Methods: Different concentrations of co-trimoxazole and rifampicin were used for loading onto a nanostructure of synthesized monomethoxy poly(ethylene glycol)-oleate (mPEG-OA). The solubility, cytotoxicity, and efficacy of these nano-packed antibiotics on Brucella-infected murine phagocytic cells were examined, as compared with free antibiotics. Then the release  nanoparticles was increased approximately 3.5 and 1.5fold, respectively, which is considerable in comparison with free insoluble ones. Despite acceptable loading percentage, the application of co-trimoxazole-loaded nanoparticle on Brucella-infected J774A.1 murine macrophage-like cells did not lead to reduction in the number of bacteria; however, the efficacy of rifampicin on Brucella-infected murine phagocytic cells was enhanced. Conclusion: In the current study, the efficacy of rifampicin on reducing the number of Brucella melitensis was increased by the novel synthesized nanostructure. In contrast, since co-trimoxazole efficacy was not enhanced by loading onto nanoparticles, the co-trimoxazole inefficiency is most likely not due to its low penetration or insolubility, and probably there are other factors that remain to be clarified in the future investigations.
کلیدواژه‌های انگلیسی مقاله Brucellosis, Rifampicin, Cytotoxicity, Nanoparticles
نویسندگان مقاله | Narges Bodaghabadi
Molecular Genetics Department, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran


| Samira Hajigholami
Molecular Genetics Department, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran


| Ziba Vaise Malekshahi
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Maliheh Entezari
Department of Biology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran


| Farhood Najafi
Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran


| Hadi Shirzad
Medical Genetic Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Majid Sadeghizadeh
Molecular Genetics Department, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-697&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-590021.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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