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Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۲، صفحات ۱۷۵-۱۸۰
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عنوان انگلیسی Ascorbic acid augments colony spreading by reducing biofilm formation of methicillin resistant Staphylococcus aureus
چکیده انگلیسی مقاله Objective(s):Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance andversatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascorbic acid on biofilm formation and colony spreading processes of S. aureus and MRSA. Materials and Methods: The isolates of methicillin-resistant S. aureus (MRSA) used in present study, were recovered from different food samples. Various selective and differential media were used for identification and confirmation of S. aureus. Agar dilution method was used for determination of oxacillin and ascorbic acid resistance level. MRSA isolates were re-confirmed by E-test and by amplification of mecA gene. Tube methods and Congo-Red agar were used to study biofilm formation processes. Gene expression studies were carried on real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results: The results revealed the presence of mecA gene belonging to SCCmecA type IV along with agr type II in the isolates. In vitro studies showed the sub-inhibitory concentration of oxacillin induced biofilm production. However, addition of sub-inhibitory dose of ascorbic acid was found to inhibit EPS production, biofilm formation and augment colony spreading on soft agar plates. The inhibition of biofilm formation and augmentation of colony spreading observed with ascorbic acid alone or in combination with oxacillin.  Moreover, gene expression studies showed that ascorbic acid increases agr expression and decreases icaA gene expression. Conclusion:The present study concluded thatascorbic acid inhibits biofilm formation, promotes colony spreading and increases agr gene expression in MRSA.
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نویسندگان مقاله zulfiqar میرانی | zulfiqar mirani
fmrrc, microbiological analytical centre, pakistan council of scientific and industrial research laboratories complex, karachi


محمد خان | muhammad khan
fmrrc, microbiological analytical centre, pakistan council of scientific and industrial research laboratories complex, karachi


anila صدیقی | anila siddiqui
fmrrc, microbiological analytical centre, pakistan council of scientific and industrial research laboratories complex, karachi


فوزیه خان | fouzia khan
dow university of medical and health science karachi


mubashir عزیز | mubashir aziz
department of veterinary pathology, microbiology section, bzu multan-pakistan


shagufta ناز | shagufta naz
fmrrc, microbiological analytical centre, pakistan council of scientific and industrial research laboratories complex, karachi


ایاز احمد | ayaz ahmed
pcmd, iccbs, university of karachi, karachi-75270. pakistan


seema خان | seema khan
fmrrc, microbiological analytical centre, pakistan council of scientific and industrial research laboratories complex, karachi
نشانی اینترنتی http://ijbms.mums.ac.ir/article_10063.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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