این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۹، شماره ۵، صفحات ۲۸۳-۲۸۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی New Delhi metallo-β-lactamase-1–producing Klebsiella pneumoniae isolates in hospitalized patients in Kashan, Iran
چکیده انگلیسی مقاله Background and Objectives: New Delhi metallo-ß-lactamase (NDM) is a newly emerging metallo-ß-lactamases, which can destroy all β-lactams including carbapenems. Therefore, this study aimed at evaluating New Delhi metallo-ß-lactamase-1–production in clinical isolates of Klebsiella pneumoniae in Kashan, Iran. Materials and Methods: In a cross-sectional study, 181 K. pneumoniae isolates were collected from clinical samples of patients, who referred to Shahid Beheshi hospital in Kashan during November 2013 and October 2014. Antimicrobial susceptibility patterns were determined using disk diffusion method, according to CLSI guidelines. Metallo-ß-lactamase (MBL) production was identified among imipenem- resistant K. pneumoniae isolates using imipenem-EDTA double disk synergy test (EDTA-IMP DDST). PCR method and sequencing were used to detect integron Class 1 and bla NDM-1 gene. Statistical analyses were performed using SPSS software Version 16. Results: Of the 181 K. pneumoniae isolates, 36 (19.9 %) were imipenem-resistant strains. A total of 28 out of 36 (77.7%) imipenem- resistant K. pneumoniae isolates were identified as MBL producer strains. Also, 150 (82.9%) K. pneumoniae isolates carried intI1 gene, and 20 (11.1%) K. pneumoniae isolates harbored bla NDM-1 gene. Conclusion: Our study revealed a high frequency of MBL production and the presence of bla NDM-1 among K. pneumoniae strains, especially among hospitalized patients, which is alarming. Moreover, the presence of Class 1 integrons in all multi-drug resistant K. pneumoniae isolates highlights the risk of rapid spread of the resistance genes, especially in clinical settings.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله فرزانه فیروزه | farzaneh firoozeh
department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

زینب محلوجی | zeinab mahluji
department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

احسانه شمس | ehsaneh shams
department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

احمد خورشیدی | ahmad khorshidi
evidence-based phytotherapy amp; complementary medicine research center, alborz university of medical sciences, karaj, iran; department of parasitology and mycology, school of medicine, alborz university of medical sciences, karaj, iran


محمد زیبایی | mohammad zibaei


نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1439
فایل مقاله دریافت فایل مقاله
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات