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Journal of Genetic Resources، جلد ۲، شماره ۲، صفحات ۹۳-۹۷
عنوان فارسی
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عنوان انگلیسی Identification of the first Transgenic Aquatic Animal in Iran By PCR-Based Method and Protein Analysis
چکیده انگلیسی مقاله In the recent years, there is evidence of training a red type of zebrafish which differs from wild-type in body color. There is not any document how it reaches to the ornamental fish farms of Iran but at first, it was a doubt it belongs to a morphotype or genetic modification (GM). First of all, a set primer was designed to validate zebrafish species. Mitochondrial 16srDNA was selected and amplified. The validation of 16srDNA gene sequences data detected three haplotypes deposited in the GenBank. By seeking scientific articles about transgenic zebrafish, the first attention went to Glowfish (color transgenic zebrafish). We decided to build a foundation of PCR based detection for finding a gene correspondence red color of zebrafish. This sequence is specific for producing a red color in the sea anemone and it was not in genome database of wild zebrafish. A set primer was designed according to the red color of sea anemone (dsRed) to make a fragment about 680 bp in red zebrafish and cream zebrafish specimens (they had red parents). Fragment sequencing (ds/Red) revealed that it belongs to the sea anemone and none of the samples nucleotides differ from each order and positive sequence from a plasmid containing dsRed. Also, protein samples from cream and red zebrafish were extracted and evaluated with fluorescence microscope by TxRed control color. A dark and a red spread was detected for protein smear of the cream and redfish respectively. A dark spread was detected for cream fish protein smear but a red smear was marked for redfish protein. This research simply demonstrates the existence of transgenic zebrafish in Iranian ornamental fish industry.
کلیدواژه‌های انگلیسی مقاله Zebrafish, 16s rDNA gene, ds/Red gene, Genetic modification
نویسندگان مقاله شیرین جمشیدی |


سیده آزاده جوادی تکلیمی | seyedeh azadeh javadi taklimi


پیام پتکی |


رامین صیقلانی |


نشانی اینترنتی http://sc.journals.umz.ac.ir/article_1658_1096a871a284e1d574f5147d2e708f44.pdf
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زبان مقاله منتشر شده en
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