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Iranian Journal of Microbiology، جلد ۹، شماره ۴، صفحات ۲۳۴-۲۴۳

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عنوان انگلیسی Isolation and characterization of probiotics from dairies
چکیده انگلیسی مقاله Background and Objectives: Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. LAB group can be isolated and characterized from traditional dairy sources. This study aimed at isolating, identifying, and in vitro characterizing (low pH/high bile salt tolerance, antibacterial activity, and antibiotic susceptibility) LAB strains from traditional Iranian dairy products. Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, ARDRA, and 16S rDNA gene sequencing. Results: A total of 19 LAB strains belonging to 4 genera ( Lactococcus, Leuconostoc, Lactobacillus and Enterococcus ) were identified. Conclusion: The experiments revealed that L. plantarum 15HN, L. lactis subsp. cremoris 44L and E. mundtii 50H strains, which were isolated from shiraz, cheese and shiraz, respectively, displayed a desirable tolerance to low pH and high bile salts, favorable anti-pathogen activity, and acceptable antibiotic susceptibility; hence, they could be considered as novel probiotic candidates and applied in the food industry.
کلیدواژه‌های انگلیسی مقاله Fingerprinting, Probiotic, Dairy products, Cheese, Shiraz

نویسندگان مقاله بابک حق شناس | babak haghshenas
departmen of food biotechnology, branch for northwest amp; west region, agricultural biotechnology research institute of iran, agricultural research, education and extension organization areeo , tabriz, iran


یوسف نامی | yousef nami
university of medical sciences, department of health and social medicine, kermanshah, iran


علی الماسی | ali almasi
chemical and environmental engineering department, faculty of engineering, university putra malaysia, 43400 upm serdang, selangor, malaysia


norhafizah عبدالله | norhafizah abdullah
chemical and environmental engineering department, faculty of engineering, university putra malaysia, 43400 upm serdang, selangor, malaysia


dayang radiah | dayang radiah
institute of biosciences, university putra malaysia, 43400 upm serdang, selangor, malaysia


رزیتا rosli | rozita rosli
biotechnology research center, department of pharmacognosy, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

ابوالفضل برزگری | abolfazl barzegari
biotechnology research center, department of pharmacognosy, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

احمد یاری خسروشاهی | ahmad yari khosroushahi



نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/356
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زبان مقاله منتشر شده en
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