| چکیده انگلیسی مقاله |
Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cells (Caco-2) cells. Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity were assessed by Western blot analysis and fluorescent assay, respectively. Results: Our results confirmed that the amount of IDE was higher in cell extract compared to supernatant, and different passage numbers and culture times had small effect on IDE expression. However, when cells were cultured in the passage number range of 5-15 for 72 hours, the IDE activity was 35% higher compared to other passage numbers (P< 0.05). Conclusion: The use of Caco-2 cells at passage number range of 5-15 and culture time of 72 hours provides proper conditions for the studies related to IDE. |
| نویسندگان مقاله |
طیبه محمدی فارسانی | taiebeh mohammadi farsani
department of medical biotechnology, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
الهه متوسلی | elahe motevaseli
department of molecular medicine, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran, food microbiology research center, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
نادیا نیازی | nadia neyazi
department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, international campus tums- ic , 88 italia st., tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمدرضا خرمی زاده | mohammad reza khorramizadeh
biosensor research center, endocrinology and metabolism molecular-cellular sciences institute, tehran university of medical sciences, tehran, iran. department of medical biotechnology, school of advanced technologies in medicine, tehran university of medi
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
الهه ظفرواحدیان | elaheh zafarvahedian
department of pharmacology and toxicology, faculty of pharmacy, tehran university of medical sciences, tehran, iran; tehran, iran,
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمد حسین قهرمانی | mohammad hossein ghahremani
department of molecular medicine, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. department of pharmacology and toxicology, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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