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Iranian Journal of Microbiology، جلد ۳، شماره ۴، صفحات ۲۱۶-۲۲۱
عنوان فارسی
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عنوان انگلیسی Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
چکیده انگلیسی مقاله Background and Objectives : Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of vitamin A, or a pigment involved in the visual attraction of animals as mating partners. The impact of gamma irradiation was studied on the Phaffia rhodozyma genome. Materials and Methods: Ten mutant strains, designated Gam1-Gam10, were obtained using gamma irradiation. Ten decamer random amplified polymorphic DNA (RAPD) primers were employed to assess genetic changes. Results : Nine primers revealed scorable polymorphisms and a total of 95 band positions were scored; amongst which 38 bands (37.5%) were polymorphic. Primer F with 3 bands and primer J20 with 13 bands produced the lowest and the highest number of bands, respectively. Primer A16 produced the highest number of polymorphic bands (70% polymorphism) and primer F showed the lowest number of polymorphic bands (0% polymorphism). Genetic distances were calculated using Jaccard's coefficient and the UPGMA method. A dendrogram was created using SPSS (version 11.5) and the strains were clustered into four groups. Conclusion : RAPD markers could distinguish between the parental and the mutant strains of P. rhodozyma. RAPD technique showed that some changes had occurred in the genome of the mutated strains. This technique demonstrated the capability to differentiate between the parental and the mutant strains.
کلیدواژه‌های انگلیسی مقاله astaxanthin,Phaffia rhodozyma,RAPD,UPGMA
نویسندگان مقاله نصرت اله نجفی | n najafi
women research centre, biomedical department, alzahra university, tehran, iran and agricultural biotechnology department, faculty of engineering, imam khomeini international university, qazvin, iran.

سازمان اصلی تایید شده: دانشگاه بین المللی امام خمینی (Imam khomeini international university)

حسینی شفیع | r hosseini
women research centre, biomedical department, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

ar احمدی | ar ahmadi
women research centre, biomedical department, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/116
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