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Iranian Journal of Microbiology، جلد ۵، شماره ۱، صفحات ۴۸-۵۵
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عنوان انگلیسی Molecular characterization of class 1 integrons and gene cassettes in multidrug resistant (MDR) Klebsiella spp. isolated from hospitalized and outpatients in Iran, 2009.
چکیده انگلیسی مقاله Background and objectives: Klebsiella species are of the most common bacteria involved in nosocomial and urinary tract infections. Genetic elements such as class 1 integrons have an important role in the resistance development. In this study, the share of class 1 integrons, the genetic characterization of the integron cassettes and PFGE profiles of the clinical Klebsiella isolates are evaluated in Besat University hospital of Sanandaj, Iran. Methods: Isolates from 17890 clinical specimens were identified by API20E. Antibiotic susceptibility testing and MIC were done for MDR isolates. For investigating class 1 integrons and gene cassettes, PCR by intI1 integrase and 5΄-CS/3΄-CS were performed. Integrated gene cassettes were analyzed by PCR-RFLP and sequencing. Pulsed-Field Gel Electrophoresis was carried out for studying of clonality outbreak of isolates. Results: Thirty five Klebsiella spp . were isolated and included 29 K. pneumoniae and six K. oxytoca . All the isolates were susceptible to carbapenems while other antibiotics showed high resistant profile. In all Klebsiella spp . PCR for intI1 integrase and 5΄-CS/3΄-CS were positive (100%). Sequencing for prevalent bands of internal variable regions between 5΄-CS/3΄-CS showed arr-5 , orfD-aacA4 and aad5- dfrA17 . PFGE Analysis showed 18 clusters in K. pneumoniae with clonality relatedness in some cases but no relatedness among K. oxytoca isolates. Conclusion: High prevalence of class 1 integron carrying gene cassettes confirms that integron-mediated antimicrobial gene cassettes are important in Klebsiella spp. resistance profile. Clone diffusions of MDR Klebsiella spp. which harbor class 1 integrons have threaten the potential in the resistance development in our clinical settings.
کلیدواژه‌های انگلیسی مقاله Klebsiella spp.,class 1 integrons,multidrug resistante (MDR)
نویسندگان مقاله هیمن سلیمی زند | himen salimizand
department of microbiology, school of medicine, kurdistan university of medical sciences, sanandaj, iran.


فرشته شاهچراغی | fereshteh shahcheraghi
department of microbiology and microbiology research center, pasteur institute, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

عنایت الله کلانتر | enayatollah kalantar
department of microbiology, school of medicine, kurdistan university of medical sciences, sanandaj, iran.


فرزاد بادمستی | farzad badmasti
department of microbiology and microbiology research center, pasteur institute, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/653
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