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Iranian Journal of Microbiology، جلد ۶، شماره ۵، صفحات ۳۱۷-۳۲۳
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عنوان انگلیسی Immunogenicity comparison of conjugate vaccines composed of alginate and lipopolysaccharide of Pseudomonas aeruginosa bound to diphtheria toxoid.
چکیده انگلیسی مقاله Background and Objectives: Treatment of Pseudomonas aeruginosa infections is greatly hampered by innate and acquired antibiotic resistance. The goal of this study was to compure the immunogenicity of conjugates of P. aeruginosa depolymerized alginate-diphtheria toxoid (D-ALGDT) and P. aeruginosa detoxified lipopolysaccharidediphtheria toxoid (D-LPSDT) in mouse model. Materials and Methods: Alginate and LPS were purified from P. aeruginosa strain PAO1. The resulting depolymerized alginate (D-ALG) and detoxified LPS (D-LPS) were covalently coupled to diphtheria toxoid (DT) as a carrier protein with adipic acid dihydrazide (ADH) as a spacer molecule and carbodiimide as a linker. Sterility, safety and pyrogenicity tests were performed. 30 mice in two groups were immunized intraperitoneally on days 0, 14 and 28 with 10 µg of D-ALGDT and D-LPSDT. Conjugates specific antibody levels were also determined by enzyme-linked immunosorbent assay (ELISA). Results: The conjugates were non-toxic and non-pyrogenic. Conjugates of D-ALGDT and D-LPSDT were shown to be safe and to elicit total IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3 antibodies in mice. ELISA results indicated that antibodies titer of D-ALGDT was more than D-LPSDT. Conclusion: Immunization with D-ALGDT showed significant increase in all types of antibodies titers in versus D-LPSDT,suggesting D-ALGDT as a vaccine candidate against P. aeruginosa infections.
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نویسندگان مقاله فایزه نجف زاده | faezeh najafzadeh
department of microbiology, zanjan branch, islamic azad university, zanjan, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

غزاله جابری | ghazaleh jaberi
department of microbiology, science and research branch, islamic azad university, kerman, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

دکتر رضا شاپوری | reza shapouri
biologic research center, zanjan branch, islamic azad university, zanjan, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

مهدی رهنما | mehdi rahnema
biologic research center, zanjan branch, islamic azad university, zanjan, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

اشرف کریمی نیک | ashraf karimi nik
department of microbiology, science and research branch, islamic azad university, kerman, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

انورسادات کیان مهر | anvarsadat kianmehr
medical biotechnology department, school of advanced medical sciences, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/378
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