این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۷، شماره ۱، صفحات ۳۸-۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Enterobacterial repetitive intergenic consensus (ERIC) PCR based genetic diversity of Xanthomonas spp. and its relation to xanthan production
چکیده انگلیسی مقاله Background and Objective : The genus Xanthomonas is composed of phytopathogenic bacterial species. In addition to causing crops diseases, most of the Xanthomonas species especially Xanthomonas campestris produce xanthan gum via an aerobic fermentation process. Xanthan gum is, an important exopolysaccharide from Xanthomonas campestris, mainly used in the food, petroleum and other industries. the purpose of this study was assessment of relationship between genetic diversity and xanthan production in Xanthomonas spp. Materials and Methods : In this study 15 strains of Xanthomonas spp. which had previously been isolated from soils of vegetable farms, were discriminated from each other using Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and 16S rDNA sequencing methods. Xanthan production of strains was measured in 250 ml flask. The results of ERIC PCR and xanthan production was compared. Results : ERIC-PCR patterns not only could differentiate all Xanthomonas campestis from the control i.e. Xanthomonas translucent but also discriminate strains of Xanthomonas to three clusters with 40% similarity based on Jaccard’s coefficient. This clustering of the strains was in agreement with other characteristics including xanthan production and biochemical features. Discussion : The results showed that genomic fingerprinting conferred adequate genetic data for discriminating between strains of the species Xanthomonas campestris. The data indicated a partial relationship between ERIC-PCR patterns and xanthan production by the strains. Conclusion : Further development of experiments may result in making good prediction about xanthan production capability of the Xanthomonas strains on the basis of ERIC-PCR method.
کلیدواژه‌های انگلیسی مقاله Genetic diversity,Xanthomonas,Xanthan,ERIC-PCR
نویسندگان مقاله عزت عسگرانی | ezat asgarani
faculty of biological sciences, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

طاهره قشقایی | tahereh ghashghaei
faculty of biological sciences, alzahra university, tehran, iran and national laboratory of industrial microbiology, department of biology, faculty of science, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

محمدرضا صعودی | mohammadreza soudi
national laboratory of industrial microbiology, department of biology, faculty of science, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نیره علی مددی | nayyereh alimadadi
national laboratory of industrial microbiology, department of biology, faculty of science, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/140
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات