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Iranian Journal of Microbiology، جلد ۷، شماره ۴، صفحات ۱۹۱-۱۹۷
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عنوان انگلیسی Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
چکیده انگلیسی مقاله Background and Objectives: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms. Materials and Methods: In this study we screened 140 diarrheatic calves (1-15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B phylotypes. Results: From diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B phylotype using DNA Microarray technology. Of these isolates, 20 contained stx a and stx b and one harbored all mentioned variants of stx genes except stx b. Conclusion: This study showed that in Tehran suburb, the B phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts.
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نویسندگان مقاله حمید استاجی | hamid staji
department of pathobiology, faculty of veterinary medicine, semnan university, semnan, iran.

سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

alfreda tonelli | alfreda tonelli
istituto zooprofilattico sperimentaledell amp;amp; 039;abruzzo e del molise amp;amp;quot; g. caporale amp;amp;quot;, research and development, campo boario, 64100 teramo, italy.


عباس جواهری وایقان | abbas javaheri vayeghan
department of pathobiology, faculty of veterinary medicine, semnan university, semnan, iran.

سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

عماد چنگیزی | emad changizi
department of pathobiology, faculty of veterinary medicine, semnan university, semnan, iran.

سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

محمدرضا سلیمی بجستانی | mohammadreza salimi bejestani
department of pathobiology, faculty of veterinary medicine, semnan university, semnan, iran.

سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/131
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