Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۵، شماره ۵، صفحات ۴۲۰-۴۲۵
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عنوان انگلیسی Identification of HLA Class I Misreads/Dropouts using serological typing, in comparison with DNA-based typing
چکیده انگلیسی مقاله Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was done by complement-dependent lymphocytotoxicity while DNA-based typing was done with sequence specific primers (SSP). Serology identified 167 HLA A and 165 HLA B antigens while SSP in same samples identified 181 HLA A and 184 HLA B alleles. A11 and B51 were the commonest antigens/alleles by both methods. There were a total of 21 misreads and 32 dropouts on serology, for both HLA A and B loci with HLA A32, B52 and B61 being the most ambiguous antigens. Inherent limitations of serological techniques warrant careful interpretation or use of DNA-based methods for resolution of ambiguous typing.
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نویسندگان مقاله حمید nawaz tipu | hamid nawaz tipu
department of immunology, armed forces institute of pathology, rawalpindi, pakistan


محمد mukarram بشیر | muhammad mukarram bashir
department of immunology, armed forces institute of pathology, rawalpindi, pakistan


محمد noma | muhammad noma
department of immunology, armed forces institute of pathology, rawalpindi, pakistan
نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/784
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Brief Communication
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