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Iranian Journal of Basic Medical Sciences، جلد ۱۰، شماره ۴، صفحات ۲۱۶-۲۲۱
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عنوان انگلیسی Comparison of Multiplex PCR and Acid Fast and Auramine -Rhodamine Staining for Detection of Mycobacterium tuberculosis and Non tuberculosis Mycobacteria in Paraffin- Embedded Pleural and Bronchial Tissues with Granulomatous Inflammation and Caseous Necrosis
چکیده انگلیسی مقاله Objective The aim of this study was to compare the sensitivity and specificity of Acid fast and Auramine-Rhodamine staining and Multiplex PCR for the detection of Mycobacterium tuberculosiscomplex and non tuberculosis Mycobacteria on formalin fixed paraffin embedded tissues (FFPE) Materials and Methods Forty cases of FFPE pleural and bronchial tissue with chronic granulomatous inflammation and caseous necrosis and 10 cases with bronchogenic carcinoma as controls were investigated. We designed a Multiplex PCR DNA amplification method with two targets: 123bp DNA fragment from IS6110, which is present only in mycobacterium tuberculosis complex and 162bp DNA encoding Ag 85complex which is present in all of mycobacteria. The FFPE also stained by Acid fast and Rhodamine-Auramine staining method. Results In 26 samples (65%) 123 bp and 162 bp DNA fragments were detected together (12 in bronchial samples and 14 in pleural samples).The 162 bp fragment wasn't detected alone. The sensitivity of PCR was 65% and the specificity was 100%. Eleven cases were positive for Acid fast staining. There was 27.5% sensitivity and 100% specificity. Thirteen cases were positive for Auramine-Rhodamine staining (A-R-S); there was 32.5% sensitivity and 100% specificity. All of the 10 controls were negative for 123 bp, 162 bp DNA fragments, for Acid fast and Auramine-Rhodamine staining. Conclusion Multiplex PCR is a sensitive, specific and rapid method for detection of M. tuberculosis in FFPE tissues.
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نویسندگان مقاله امیرحسین جعفریان | amir hossein jafarian
department of pathology, ghaem hospital, ahmadabad blvd., mashhad university of medical sciences mums mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

عباسعلی امیدی | abbasali omidi
department of pathology, ghaem hospital, ahmadabad blvd., mashhad university of medical sciences mums mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

جواد قناعت | javad ghenaat
department of microbiology, ghaem hospital, ahmadabad blvd., mums, mashhad, iran


کیارش قزوینی | kiarash ghazvini
department of microbiology, ghaem hospital, ahmadabad blvd., mums, mashhad, iran


حسین آیت اللهی | hossein ayatollahi
department of biochemistry, ghaem hospital, ahmadabad blvd., mums, mashhad, iran


مینو عرفانیان | minoo erfanian
department of pathology, ghaem hospital, ahmadabad blvd., mashhad university of medical sciences mums mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

محمد تقی شاکری | mohammadd taghi shakeri
department of microbiology, ghaem hospital, ahmadabad blvd., mums, mashhad, iran


محمود باقری | mahmoud bagheri
department of microbiology, ghaem hospital, ahmadabad blvd., mums, mashhad, iran


هومن توسلیان | hooman tavassolian
department of pathology, ghaem hospital, ahmadabad blvd., mashhad university of medical sciences mums mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_5233.html
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