این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۱۱، شماره ۳، صفحات ۱۹۰-۱۹۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Synergistic Effect of LPS, IFN- and Iron on Apoptosis of Balb/c Mice Macrophages Following Nitric Oxide Production
چکیده انگلیسی مقاله Objective(s) Previous studies have demonstrated that the nitric oxide (NO) dependent death of murine peritoneal macrophages activated in vitro with IFN-g and LPS is mediated through apoptosis. In the present study, we investigated the synergistic effect of LPS, IFN-g and iron on NO production and apoptosis. Materials and Methods After determination of iron cytotoxicity, the peritoneal macrophages of Balb/c mice were cultured with iron, LPS, and IFN-g separately, or a mixture of these for 18 hr at 37 ◦C. Then after 18 hr incubation, the level of NO in supernatant was measured by the Griess method. At the same time, after incubation with ethidium bromide and acridine orange dye, the apoptotic macrophages were detected by fluorescence microscopy. Results NO production was significantly greater than the control group in macrophages exposed to iron,LPS, or IFN-g alone (P=0.02), while no significant difference was detected in apoptosis rate in the presence of LPS (P=0.08). However, the differences were remarkable between NO production and apoptosis rate in the presence of iron, LPS and IFN-g (P≤0.05). Conclusion These findings indicate the immunostimulatory effect of iron on NO production by IFN-g and LPS.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله لیلا پیردل | leila pirdel
college of medicine and medical sciences, islamic azad university, ardebil branch, ardebil, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

احمد زواران حسینی | ahmad zavaran hosseini
department of immunology, school of medicine, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_5230.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات