| چکیده انگلیسی مقاله |
Objective(s): Stem cell differentiation into different cell lineages depends upon several factors, cell cycle control elements and intracellular signaling elements, including P15INK4b and P16INK4a genes. Epigenetics may be regarded as a control mechanism which is affected by these factors with respect to their promoter structure. Materials and Methods: The CD34 + cord blood stem cells were purified, isolated and then expanded. The undifferentiated day genome was isolated from part of the cultured cells, and the seventh day differentiated genome was isolated from the other part after differentiation to erythroid lineage. The procedure was followed by a separate Real-Time PCR for the two genes using the obtained cDNA. The processed DNA of the former stages was used for MSP (Methylation Specific PCR) reaction. Finally, pre- and post differentiation results were compared. Results: After performing MSP for each gene, it became clear that P15INK4b gene has undergone methylation and expression in predifferentiation stage. In addition, its status has not been changed after differentiation. P15INK4b gene expression was reduced after the differentiation. The other gene, P16INK4a, showed no predifferentiation methylation. Itwas completely expressed methylated and underwent reduced expression after differentiation. Conclusion : Specific predifferentiation expression of P15INK4b and P16INK4a genes along with reduction in their expression after erythroid differentiation indicated animportant role for these two genes in biology of CD34+ cells in primary stages and before differentiation. In addition, both genes are capable of epigenetic modifications due to the structure of their promoters. |
| نویسندگان مقاله |
مهدی آزاد | mehdi azad
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
سعید کاویانی | saeid kaviani
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
مهرداد نوروزی نیا | mehrdad noruzinia
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
یوسف مرتضوی | yousef mortazavi
hematology department, zanjan medical sciences university, zanjan, iran
ناصر مبرا | naser mobarra
department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. 7students’ scientific research center, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
شعبان علیزاده | shaban alizadeh
4department of hematology, allied medical school, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمد شاه جهانی | mohammad shahjahani
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
فاطمه اسکندری | fatemeh skandari
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
محمد حسین احمدی | mohammad hosein ahmadi
iranian blood transfusion organizations, medical departmen
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
امیر آتشی | amir atashi
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
سعید آبرون | saeid abroun
1hematology department, school of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
زهرا ذنوبی | zahra zonoubi
6department of obstetrics and gynecology, mahdiyeh hospital, shahid beheshti university,tehran, iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
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