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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۴، صفحات ۲۵۶-۲۶۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Spectrofluorimetric determination of cefixime using terbium-danofloxacin probe |
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| چکیده انگلیسی مقاله |
Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx. Materials and Methods: Cfx was detected based on fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. The quenched fluorescence intensity of Tb3+- Dano system is proportional to the concentration of Cfx. The optimum conditions for the determination of Cfx were studied. Results: The maximum response was achieved under optimum conditions of [Tris buffer]= 0.008 mol/l (pH 6.5), [Tb3+]=1×10-4 mol/l and [Dano]=1×10-4 mol/l. The developed method was evaluated in terms of accuracy, precision and limit of detection. The linear concentration ranges for quantification of Cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (S/N=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. The Cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory. Conclusion: This method is simple, practical and relatively interference-free for determination of Cfx in pharmaceutical tablets and serum samples. |
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| نویسندگان مقاله |
جمشید l منظوری | jamshid l manzoori
department of analytical chemistry, faculty of chemistry, university of tabriz, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
محمد امجدی | mohammad amjadi
tuberculosis and lung disease research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
ناصر سلطانی | naser soltani
tuberculosis and lung disease research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
ابوالقاسم جویبان | abolghasem jouyban
drug applied research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_2583.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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