Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۶، صفحات ۳۹۶-۴۰۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein
چکیده انگلیسی مقاله Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western blot and other assay systems. Materials and Methods: A synthetic peptide derived from β-actin protein was designed and conjugated to Keyhole limpet hemocyanin (KLH (and used to immunize a white New Zealand rabbit. The antibody was purified from serum by affinity chromatography column. The purity of the antibody was determined by SDS-PAGE and its ability to recognize the immunizing peptide was measured by ELISA. The reactivity of the antibody with β-actin protein in a panel of different cell lysates was then evaluated by western blot. In addition, the reactivity of the antibody with the corresponding protein was also evaluated by Immunocytochemistry and Immunohistochemistry in different samples. Results: The antibody could recognize the immunizing peptide in ELISA. It could also recognize β-actin protein in western blot as well as in immunocytochemistry and immunohistochemistry. Conclusion: Our data suggest that this antibody may be used as an internal control in western blot analyses as well as in other immunological applications such as ELISA,immunocytochemistry and immunohistochemistry.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله نازیلا امینی | nazila amini
department of biology, science and research branch, islamic azad university, tehran, iran 2 monoclonal antibody research center, avicenna research institute, acecr, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

محدثه نقی ویشته | mohadeseh naghi vishteh
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


امید زارعی | omid zarei
department of pharmaceutical biotechnology, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

رضا هادوی | reza hadavi
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


نگاه احمدوند | negah ahmadvand
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


حجت اله ربانی | hodjattallah rabbani
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


محمود جدی تهرانی | mahmood jeddi tehrani
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_2922.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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