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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱، صفحات ۴۷-۵۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In silico and in vitro studies of cytotoxic activity of different peptides derived from vesicular stomatitis virus G protein |
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| چکیده انگلیسی مقاله |
Objective(s):This study aims at exploring cytotoxic activity of different peptides derived from VSVG protein against MCF-7 and MDA-MB-231 breast cancer cell lines and human embryonic kidney normal cell (HEK 293). Materials and Methods: The ANTICP web server was used to predict anticancer peptides. The cytotoxic activity of peptides with high score (P26, P7) and low score (P19) was examined by MTT and DNA fragmentation assays. Results: The results obtained from ANTICP web serverdemonstrated that 4 out of 48 peptides (P26, P7, P10, and P16) had anticancer activity. P26 and P7 peptides of these 4 peptides were detected to have high cytotoxic activity against MCF-7 cells with CC50 values of 98,280 µg/ml and MDA-MB231 cells with CC50 100,550 µg/ml, respectively. In addition, the results showedthat amino acid residues of these 4 peptides were located near fusion domain. Conclusion: The results confirmed that P26 and P7 peptides might induce membrane damage and initiate apoptosis. The present study suggested that P26 and P7 peptides could be appropriate candidates for further studies as cytotoxic agents and modifications in the residue at positions 70-280 might potentially produce a more efficient VSVG protein in gene therapy. |
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| کلیدواژه‌های انگلیسی مقاله |
ANTICP, Apoptosis, Cytotoxic, Pseudo typing, VSVG protein |
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| نویسندگان مقاله |
فرشته قندهاری | fereshte ghandehari
department of biology, science and research branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
ماندانا بهبهانی | mandana behbahani
department of biotechnology, faculty of advanced sciences and technologies, university of isfahan, iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)
عباسعلی پورآذر | abbasali pourazar
department of immunology, isfahan university of medical science, isfahan, iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)
زهرا نورمحمدی | zahra noormohammadi
department of biology, science and research branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_3888.html |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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