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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۷، صفحات ۷۰۵-۷۰۹
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عنوان انگلیسی MiR-125b inhibits stromal cell proliferation in giant cell tumor of bone by targeting parathyroid hormone 1 receptor
چکیده انگلیسی مقاله Objective(s):miR-125b has been identified as a tumor suppressor in many tumors, but its role in giant cell tumor (GCT) of bone remains poorly understood. The current study aimed to investigate the potential role and mechanism of miR-125b in GCT. Materials and Methods:Expression levels of miR-125b in GCT tissues were determined using RT-PCR. The cell proliferation was surveyed by direct cell counting, MTS and CCK-8, and the apoptotic cells were evaluated by Annexin V-FITC and propidium iodine staining assay. The target gene expression was determined using RT-PCR and western blot. Parathyroid hormone 1 receptor (PTH1R) 3’-UTR was cloned into luciferase reporter plasmid to confirm direct targeting. Results: We found that miR-125b was significantly down-regulated in GCT tissues. Using both gain- and loss-of-function analyses, we further revealed that miR-125b suppressed GCT stromal cell proliferation and induced cell apoptosis. Furthermore, we revealed that PTH/PTHrP type 1 receptor is a direct and functional target of miR-125b. Conclusion:Our results suggest that miR-125b acts as a tumor suppressor through suppression of the PTH1R/RANKL signaling pathway. These findings contribute to our understanding of the functions of miR-125b in GCT.
کلیدواژه‌های انگلیسی مقاله Cell proliferation, GCT, miR-125b, PTH1R, RANKL
نویسندگان مقاله پانته feng wu | pan feng wu
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china


jie yu liang | jie yu liang
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china


fang yu | fang yu
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china


zheng bing zhou | zheng bing zhou
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china


ju yu تنگ | ju yu tang
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china


کانگ hua li | kang hua li
department of orthopaedics, xiang ya hospital, central south university, changsha 410008, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_4654.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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