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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۸، صفحات ۷۹۹-۸۰۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Estrogen treatment enhances neurogenic differentiation of human adipose derived stem cells in vitro |
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| چکیده انگلیسی مقاله |
Objective(s):Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues. The aim of this study is to evaluate the effects of estrogen on the proliferation and neural differentiation of human adipose derived stem cells (ADSCs) during neurogenic differentiation. Materials and Methods: Isolated human ADSCs were trans-differentiated in neural induction medium containing neurobasal medium, N2 and B27 with or without 17β-estradiol (E2) treatment. Proliferation rate and neural differentiation of human ADSCs were assessed using MTT assay, immunostaining and real time RT- PCR analysis, respectively. Results: Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P< 0.05). Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP2) was significantly higher in the E2 treated cell cultures when compared to the untreated cell cultures (P< 0.05). Conclusion: According to our findings, estrogen can promote proliferation and neuronal differentiation of human ADSCs. |
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| نویسندگان مقاله |
شهناز رضوی | shahnaz razavi
department of anatomical sciences and molecular biology, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
محمدرضا رضوی | mohamad reza razavi
molecular parasitology laboratory, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
نفیسه احمدی | nafiseh ahmadi
department of biology, islamic azad university, science and research branch, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
محمد کاظمی | mohammad kazemi
department of genetic, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_4731.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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