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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱۰، صفحات ۹۷۹-۹۸۸
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Determination of valproic acid in human plasma using dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection |
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| چکیده انگلیسی مقاله |
Objective(s): Dispersive liquid-liquid microextraction coupled with gas chromatography (GC)-flame ionization detector was developed for the determination of valproic acid (VPA) in human plasma. Materials and Methods: Using a syringe, a mixture of suitable extraction solvent (40 µl chloroform) and disperser (1 ml acetone) was quickly added to 10 ml of diluted plasma sample containing VPA (pH, 1.0; concentration of NaCl, 4% (w/v)), resulting in a cloudy solution. After centrifugation (6000 rpm for 6 min), an aliquot (1 µl) of the sedimented organic phase was removed using a 1-µl GC microsyringe and injected into the GC system for analysis. One variable at a time optimization method was used to study various parameters affecting the extraction efficiency of target analyte. Then, the developed method was fully validated for its accuracy, precision, recovery, stability, and robustness. Results: Under the optimum extraction conditions, good linearity range was obtained for the calibration graph, with correlation coefficient higher than 0.998. Limit of detection and lower limit of quantitation were 3.2 and 6 μg/ml, respectively. The relative standard deviations of intra and inter-day analysis of examined compound were less than 11.5%. The relative recoveries were found in the range of 97 to 107.5%. Finally, the validated method was successfully applied to the analysis of VPA in patient sample. Conclusion: The presented method has acceptable levels of precision, accuracy and relative recovery and could be used for therapeutic drug monitoring of VPA in human plasma. |
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| نویسندگان مقاله |
رعنا فاضلی بختیاری | rana fazeli bakhtiyari
liver and gastrointestinal diseases research center, tabriz university of medical sciences, tabriz, iran|department of analytical chemistry, faculty of chemistry, university of tabriz, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
وحید پناهی آذر | vahid panahi azar
drug applied research center, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
محمد حسین sorouraddin | mohammad hossein sorouraddin
department of analytical chemistry, faculty of chemistry, university of tabriz, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
ابوالقاسم جویبان | abolghasem jouyban
pharmaceutical analysis research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_5461.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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