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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۶، صفحات ۶۳۲-۶۳۷
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عنوان انگلیسی Time-dependent changes of autophagy and apoptosis in lipopolysaccharide-induced rat acute lung injury
چکیده انگلیسی مقاله Objective(s): Abnormal lung cell death including autophagy and apoptosis is the central feature in acute lung injury (ALI). To identify the cellular mechanisms and the chronology by which different types of lung cell death are activated during lipopolysaccharide (LPS)-induced ALI, we decided to evaluate autophagy (by LC3-II and autophagosome) and apoptosis (by caspase-3) at different time points after LPS treatment in a rat model of LPS-induced ALI. Materials and Methods: Sprague-Dawley rats were randomly divided into two groups: control group and LPS group. ALI was induced by LPS intraperitoneal injection (3 mg/kg). The lung tissues were collected to measure lung injury score by histopathological evaluation, the protein expression of LC3-II and caspase-3 by Western blot, and microstructural changes by electron microscopy analysis. Results: During ALI, lung cell death exhibited modifications in the death process at different stages of ALI. At early stages (1 hr and 2 hr) of ALI, the mode of lung cell death started with autophagy in LPS group and reached a peak at 2 hr. As ALI process progressed, apoptosis was gradually increased in the lung tissues and reached its maximal level at later stages (6 hr), while autophagy was time-dependently decreased. Conclusion:These findings suggest that activated autophagy and apoptosis might play distinct roles at different stages of LPS-induced ALI. This information may enhance the understanding of lung pathophysiology at the cellular level during ALI and pulmonary infection, and thus help optimize the timing of innovating therapeutic approaches in future experiments with this model.
کلیدواژه‌های انگلیسی مقاله Acute lung injury, Apoptosis, Autophagy, Lipopolysaccharide
نویسندگان مقاله li lin | li lin
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china|hubei province key laboratory on cardiovascular, cerebrovascular, and metabolic disorders, hubei university of science and technology, xianning


lijun زانگ | lijun zhang
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china|hubei province key laboratory on cardiovascular, cerebrovascular, and metabolic disorders, hubei university of science and technology, xianning


liangzhu yu | liangzhu yu
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china|hubei province key laboratory on cardiovascular, cerebrovascular, and metabolic disorders, hubei university of science and technology, xianning


lu هانیه | lu han
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china


wanli ji | wanli ji
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china|hubei province key laboratory on cardiovascular, cerebrovascular, and metabolic disorders, hubei university of science and technology, xianning


هیو shen | hui shen
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china


zhenwu hu | zhenwu hu
school of basic medicine, hubei university of science and technology, xianning 437100, p.r. china|hubei province key laboratory on cardiovascular, cerebrovascular, and metabolic disorders, hubei university of science and technology, xianning
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7131.html
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زبان مقاله منتشر شده en
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