| چکیده انگلیسی مقاله |
Background: Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study is to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Methods: Cells were treated by different concentrations of ATO either in presence or absence of IR. Viability and apoptosis pathway of both treated and control groups were evaluated using MTT assay and the expression analysis of Bax, Bcl-2, and caspase-3 genes, respectively. All treatments were performed on 100-μm diameter spheroids. Results: Results showed a significant reduction in the survival of the cells in all treated groups. As expected, cell survival was much less in combination treatment than treatment with only ATO. Moreover, combination therapy made Bax and caspase-3 up-regulated and Bcl-2 down-regulated. Conclusion: ATO and radiation had a synergistic apoptotic effect on GBM cells by up-regulation of caspase-3 and alteration of the Bax-Bcl-2 balance; therefore, ATO may act as a potential anti-cancer agent against GBM cells through triggering the mitochondrial pathway of apoptosis. |
| نویسندگان مقاله |
کاوه مولودی | kave moloudi
radiation sciences department, faculty of allied medicine school, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
علی نشاسته ریز | ali neshasteriz
radiation biology research center, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
ارشد حسینی | arshad hosseini
department of medical biotechnology, faculty of allied medicine, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
نازیلا عیوض زاده | nazila eyvazzadeh
radiation research center, faculty of paramedicine, aja university of medical sciences, tehran, iran
مهدی شمالی | mehdi shomali
radiology department, faculty of allied medicine, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
سمیرا عینعلی | samira eynali
medical physics and biomedical engineering department, school of medicine, tran university of medical sciences, tehran, iran
الهه میرزایی | elahe mirzaei
microbiology department, faculty of science, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
اسعد آذرنژاد | asaad azarnezhad
cellular amp; molecular research center, kurdistan university of medical sciences, sanandaj, iran
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