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Iranian Endodontic Journal، جلد ۱۲، شماره ۴، صفحات ۴۸۸-۴۹۲
عنوان فارسی
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عنوان انگلیسی In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate
چکیده انگلیسی مقاله Introduction: The present study sought to evaluate and compare biocompatibility and setting time of Retro mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) and Angelus MTA. Methods and Materials: CEM cement, Angelus MTA and Retro MTA were assessed in set and fresh states. Extracts transformed to each cavity of three 24-well plates in which 1×10 4 cell were seeded into each well 24 h earlier. All specimens were incubated in a humidified incubator with 5% CO2 at 37 ° C. Mosmann’s tetrazolium toxicity (MTT) assay was used to determine in vitro cytotoxicity on L929 mouse fibroblast cell line. Cell viability was determined at 1, 24, and 72 h after exposure. The initial setting time was measured by 113.4 g Gilmore needle testing. Then, final setting times were assessed by the 456.5 g Gilmore needle. Data comparisons were performed using the analysis of variance (ANOVA) and Tukey's post hoc test ( α =0.05). Results: All groups in both forms indicated higher cell vitality compared to positive control group ( P < 0.001). After 24 h, the set Retro MTA showed better biocompatibility compared to set CEM and set Angelus MTA ( P < 0.001). Retro MTA showed significantly lower initial and final setting time compared to CEM and Angelus MTA ( P < 0.001). Conclusion: Our results indicated the good cell viability values of Retro MTA and relatively short period of setting time. It seems a promising alternative material in clinical situations where accelerated setting is required. However, more clinical and in vivo investigations are needed for a clear decision making.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله طاهره pornamaze | tahereh pornamaze


زهرا یادگاری | zahra yadegari


امیر قاسمی | amir ghasemi


سیده مهسا شیخ al اسلامیان | seyedeh mahsa sheykh al eslamian


شیوا شجاعیان | shiva shojaeian


نشانی اینترنتی http://journals.sbmu.ac.ir/iej/article/view/16275
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/109/article-109-439069.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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