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Journal of Arthropod-Borne Diseases، جلد ۱۱، شماره ۳، صفحات ۰-۰
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عنوان انگلیسی Genetic Variation of the -tubulin Gene of Babesia caballi Strains
چکیده انگلیسی مقاله Background: Equine piroplasmosis is caused by two haemoprotozoan parasites: Babesia caballi and Theileria equi . Negative economic impact on international trade has been associated to endemic sites. This is the reason why carrier detection requires reliable diagnostic methods. Various diagnostic modalities can be used alone or in combination including PCR. However, genetic variation of commonly used genes is still of debate. The aim of this research was to sequence the b-tubulin gene of a B. caballi strain from Spain and to compare it with known b-tubulin sequences. Methods: DNA was isolated from a cryopreserved strain from Spain and acute and chronic carrier horses. Firstly, degenerated primer pairs were designed based on GenBank sequences of different Babesia and Theileria species for sequencing. The primers were redesigned to amplify both parasites, simultaneously. Finally, a species-specific pri­mer pair for B. caballi was designed and a Restriction Fragment Length Polymorphism-PCR (PCR-RFLP) assay performed to know the difference of known B. caballi strains. Results: We provided new insights of the b-tubulin gene and a good molecular coverage of this gene, contributing with a number of useful primers to amplify T. equi and B. caballi . Moreover, PCR-RFLP assays based on the exon II of this gene confirmed the causative B. caballi strain in Spanish horses. Conclusion: We reported useful primer pairs for diagnostic and a new sequence of the b-tubulin gene of B. caballi , which will facilitate the development of future assays and the detection of infected horses, preventing thus the spread of this disease worldwide.
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نویسندگان مقاله maría guadalupe montes cortés | maría guadalupe montes cortés
genetics and animal breeding, veterinary faculty, universidad de extremadura, cáceres, spain


جوز لوییس فرناندز گارسیا | josé luis fernández garcía
parasitology and parasitological diseases, veterinary faculty, universidad de extremadura, cáceres, spain


میگویل ángel habela مارتینز estéllez | miguel ángel habela martínez estéllez


نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/654
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/77/article-77-430364.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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