چکیده انگلیسی مقاله |
Background: Fungi have a worldwide distribution which can cause a broad spectrum of disease ranging from allergic to systemic infections, particularly in immunocompromised individuals. Fungal spores are an important group of bioaerosols in hospital environment which are an emerging cause of hospital-acquired infection. Nosocomial infections cause significant morbidity and mortality as well as large financial burden on the healthcare system. This study aimed to evaluate the frequency and species distribution of airborne fungi in selected wards of two tertiary hospitals in Tehran, Iran. Methods: In this cross-sectional study, samples were collected during six months from July 2016 to December 2016 by using of settle plate method. Samples were collected from selected wards of Imam Khomeini Hospital and Children's Medical Center and then incubated at 28 °C for 8-10 days. Fungal isolates were identified using the macroscopic features of colony and microscopic characteristics in slide cultures. Yeast isolates were identified by CHROMagar candida medium. PCR-sequencing of ITS1-5.8 S-ITS2 region of ribosomal DNA was used for identification of unknown isolates. Results: A total of 202 colonies including 133 colonies from Imam Khomeini Hospital and 69 colonies from Children's Medical Center were isolated. Cladosporium spp. were the most common obtained fungi accounted for 30.1% and 47.8% of all isolates in Imam Khomeini Hospital and Children's Medical Center, respectively. Penicillium spp. and Aspergillus spp. were other frequent species in two hospitals. Infectious diseases ward in Imam Khomeini hospital and emergency and urology wards in Children's Medical Center had the highest rate of contamination. Conclusion: According to the results of this study, the frequency and diversity of fungal spores in hospital wards were different. In addition, since the fungal contamination in the hospital environment are affected by various environmental factors and the efficiency of ventilation systems, some of these wards require better ventilation system as well as regular monitoring to remove these fungal bioaerosols in order to maintain the health of patients and health care workers. |
نویسندگان مقاله |
زهرا کمالی سروستانی | zahra kamali sarwestani students’ scientific research center, tehran university of medical sciences, tehran, iran مرکز پژوهش های علمی دانشجویان سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
علیرضا داسدار | alireza dasdar shahid beheshti university of medical sciences, tehran, iran دانشکده دندانپزشکی سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
ستاره آقا کوچک افشاری | setareh agha kuchak afshari department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محسن گرامی شعار | mohsen gerami shoar department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
سید جمال هاشمی | seyyed jamal hashemi department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
رضا پاکزاد | reza pakzad department of epidemiology and biostatistics, school of public health, گروه اپیدمیولوژی و آمار زیستی
پگاه آردی | pegah ardi department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
علیرضا عبدالهی | alireza abdollahi department of pathology, imam khomeini hospital complex, school of medicine, گروه پاتولوژی، مجتمع بیمارستانی امام خمینی ره ، دانشکده پزشکی
محمدتقی حقی آشتیانی | mohammad taghi haghi ashtiani - department of pathology, children amp;apos;s medical center, pediatrics center of excellence, tehran university of medical sciences, tehran, iran -گروه پاتولوژی، مرکز طبی کودکان سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
شهرام محمودی | shahram mahmoudi department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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