Journal of Kerman University of Medical Sciences، جلد ۱۱، شماره ۱، صفحات ۲۱-۳۱
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عنوان انگلیسی Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers
چکیده انگلیسی مقاله For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR with genus-specific primers of Bif164f and Bif601r and 16SrRNA gene sequence analysis were applied. Results of PCR confirmed the obtained phenotypic identifications. Moreover by this method the 8 remaining strains were identified as Bifidobacterium species. Using sequencing 16SrRNA gene, 5 B. longum strains were identified that had different fermentation pattern from B. longum. Some new ribose negative Bifidobacterium longum strains were also identified. The obtained results present new strains of Bifidobacterium longum.
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نویسندگان مقاله m خمیری |
phd. student of food science and technology

سید علی مرتضوی | s a
professor

h b قدوسی | h b
associate professor, food science and technology department, agriculturl school, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

a khamessan |
member of animal science research institute, karaj, iran

d احمد |
faculty member of inrs, canada

f شهیدی |
associate professor, food science and technology department, agriculturl school, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_32028_615236f0cc12f1da59b50901fe5a31ab.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-405281.pdf
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