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Journal of Kerman University of Medical Sciences، جلد ۱۳، شماره ۴، صفحات ۲۳۰-۲۳۹
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Role of orFD Pseudomonas aeruginosa H103 Gene in Glucose Uptake |
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| چکیده انگلیسی مقاله |
Background:Pseudomonas aeruginosa is a gram negative non facultative bacterium and one of the members of normal flora in different sites of body in healthy humans.this bacterium can resist in fluids and hospital environments for a long time.Pseudomonas aeruginosa has two systems for glucose uptake:a low affinity oxidative pathway and a high affinity phosohorylative pathway.Although the role of these genes are unknown by now,they may have a role in regulation of glucose uptake.In the present study,the role of orFD gene in glucose uptake in P.aeruginosa has been investigated. Method:orFD fragment were cloned in pUCP20 as vector and the recombinant plasmid transferred into WMA200 strain of P.aeruginose,a mutant strain of p.aeruginosa with a chromosomal deletion of orFBCD.So we compared the rats of glucose uptake by p.aeruginosa by using labeled glucose under condifions at low substrate concentration and low cell density. Results:Carbohydrate uptake patterns differed considerably among three strains.the wild type is able to uptake glucose at a faster rate than the mutant:however,the mutant complemented with orFD shows an intermediate uptake comparing to the wild type and the mutant. Conclusion:orFD gene has an important role in carbohydrate uptake in p.aeruhinosa strains however fuether studies are required to determine the involved mechanism. |
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| کلیدواژه‌های انگلیسی مقاله |
aeruginosa, Glucose Pseudomonas uptake, OrFB, OrFC, OrFD |
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| نویسندگان مقاله |
n حسینی جزنی | hoseini jazani
assistant professor
a عبدی علی | abdi ali
assistant professor
e worobec |
associate professor
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| نشانی اینترنتی |
http://jkmu.kmu.ac.ir/article_17585_d18e8a0c90e2fb1e6aa60dc96dff9c8a.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-405239.pdf |
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| زبان مقاله منتشر شده |
en |
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