این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Kerman University of Medical Sciences، جلد ۱۶، شماره ۱، صفحات ۳۲-۴۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In vitro Evaluation of Anti-Leishmanial Activities of Zataria Multiflora Boiss, Peganum Harmala and Myrtus Communis by Colorimetric Assay
چکیده انگلیسی مقاله Background & Aims: Plant extracts and plant-derived compounds are valuable sources of new medicinal agents that are commonly used to treat infectious diseases. The purpose of this study was to evaluate, antiLeishmanial activity of three plant extracts on L.major promastigotes by colorimetric assay as compared to a trivalent antimonial compound (tartar emetic). Methods: Leishmania major promastigotes were cultured at 25±2°C in stationary phase in RPMI-1640 medium supplemented with 10% heat inactivated fetal calf serum (FCS) and antibiotic. Then by using MTT assay, the biological activity of plant extracts in comparison to potassium antimonyl tartrate [Sb (III)] on L.major promastigotes were assessed. The optical density (OD) due to cleavage of the tetrazolium salt MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] into a colored product formazan by the parasite was measured using ELISA reader and IC50 values (50% inhibitory concentrations) were determined. All experiments were repeated in duplicate. Results: plant extracts and Tartar emetic inhibited the growth of promastigote forms of L.major in vitro after 72 hours of incubation. IC50 of tartar emetic was 4.7 µg/ml, and IC50 values for Zataria multiflora Boiss, Peganum harmala and Myrtus communis extracts were 7.4, 7.2 and 5.8 µg/ml, respectively. Although tartar emetic was more effective than plant extracts, all extracts had profound effects on promastigotes of L.major. Conclusion: Since, plant extracts of Zataria multiflora Boiss, Peganum harmala and Myrtus communis have anti-Leishmanial effects in vitro, further works are required to evaluate the exact effect of these extracts on Leishmania agents in animal models.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m براتی |
master in medical parasitology, leishmaniasis research center amp;amp; afzalipour school of medicine, kerman university of medical sciences, kerman, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

i شریفی |
professor of parasitology, leishmaniasis research center amp;amp; afzalipour school of medicine, kerman university of medical sciences, kerman, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

f شریفی فر |
assistant professor of pharmacognosy, school of pharmacy, kerman university of medical sciences, kerman, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_17245_b7e0cecd586cc96f78de4917bdd64af1.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-405121.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات