Journal of Kerman University of Medical Sciences، جلد ۲۳، شماره ۶، صفحات ۷۰۳-۷۱۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Effect of TLR4 Asp299Gly polymorphism on IL-6 and IL -18 expression in H.pylori infected patients
چکیده انگلیسی مقاله Background & Aims: Helicobacter pylori (H. pylori) infection is associated with peptic ulcer and gastric cancer. Polymorphisms of the genes coding Toll-like receptors (TLRs) may influence the innate and adaptive immune responses and affect the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expression in patients infected with H. pylori have remained unclear. The purpose of this study was to investigate the effect of TLR-4 Asp299Gly polymorphism on expression levels of IL-6 and IL-18 genes in patients infected with H. Pylori in comparison to uninfected patients. Methods: In a case-control study, biopsies were collected from 58 H. pylori-infected and 44 uninfected subjects.Genotypes of TLR-4 Asp299Gly single-nuleotide polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The expression levels of mucosal IL-6 and IL-18 mRNA were measured by real time-PCR. Cytokine expression levels in the two groups were analysed using the t test. Results: The frequencies of D/D and D/G genotypes were respectively 55.2% and 44.8% in H. pylori-infected and 77.2% and 22.8% in uninfected subjects. The expression level of IL-6 in H. pylori infected patients with TLR-4 gene polymorphism was significantly higher (P=0.001). However, no significant relationship between the expression level of IL-18 and TLR-4 gene polymorphism was reported in patients infected with H. pylori (P=0.419). In addition, no significant relationship between IL-6, IL-18 expression levels and TLR-4 gene polymorphism was reported in uninfected patients. Conclusion: TLR-4 Asp299Gly Gene single-nuclotide polymorphism increases the expression levels of TL-6 and has an important role in development of Hopylori-associated gastrititis.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله قربانعلی رحیمیان |
assistant professor, department of internal medicine amp; cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, iran

نادر باقری |
ph.d. student in immunology, department of immunology, school of public health amp; imonology research center, tehran university of medical, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهرکرد (Shahr kord university of medical sciences)

ابوالفضل قلی پور |
assistant professor, department of microbiology and immunology, faculty of mdicine amp; cellular and molecular research center, shahrekord university of medical sciences, shahrekord, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

هدایت اله شیرزاد |
professor, faculty of mdicine amp; cellular and molecular research center, shahrekord university of medical sciences, shahrekord, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهرکرد (Shahr kord university of medical sciences)

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_41263_7e0ab6d592caa0d0e69f8b543a02a8cd.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-404751.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات