| چکیده انگلیسی مقاله |
Background: Sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. In the current study, we investigated the modulation of Phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors. Methods: Sand flies were grouped according to physiological stages such as unfed, fed, semi-gravid, gravid, parous, nulliparous, infected or non-infected with Leishmania major and based on the season in which they were collected. Salivary gland antigens (SGAs) were analyzed using SDS-PAGE and the antibody response against SGAs in Rhombomys opimus was determined by ELISA and Western blot. Results: The highest protein content was found in the salivary glands of unfed sand flies. The saliva content was higher in parous compared to nulliparous, in summer compared to spring, and in Leishmania-infected compared to non-infected flies. The salivary gland lysate (SGL) electrophoretic pattern variations were observed among sand flies with various physiological stages particularly from 4–9 protein bands of 14–70 kDa. The SGL of unfed and gravid flies had extra protein bands compared to fed and semi-gravid sand flies. There was missing protein bands in SGL of parous compared to nulliparous; and in summer compared to spring collected flies. Rhombomys opimus serum reacted strongly with an antigenic band of around 28 kDa in the SGL of all sand fly groups. Conclusion: Certain biological and environmental characteristics of wild populations of vector sand flies affect the protein content and antigenicity of saliva. This might have an important implication in the design of vector-based vaccines. |
| نویسندگان مقاله |
نسیبه حسینی واسوکلایی | nasibeh hosseini vasoukolaei
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
احمدرضا محمودی | ahmadreza mahmoudi
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran.
علی خامسی پور | ali khamesipour
center for research and training in skin diseases and leprosy, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمدرضا یعقوبی ارشادی | mohammadreza yaghoobi ershadi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
shaden kamhawi | shaden kamhawi
laboratory of malaria and vector research, national institute of allergy and infectious diseases, national institute of health, rockville, usa.
عیسی g valenzuela | jesus g valenzuela
laboratory of malaria and vector research, national institute of allergy and infectious diseases, national institute of health, rockville, usa.
محمدحسین آرندیان | mohammadhossein arandian
esfahan health research station, national institute of health research, tehran university of medical sciencesesfahan, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
حسین میرهندی | hossein mirhendi
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
شقایق امامی | shaghayegh emami
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran.
زهرا سعیدی | zahra saeidi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
فرح idali | farah idali
reproductive immunology research center, avicenna research institute, acecr, tehran, iran.
رضا جعفری | reza jafari
esfahan health research station, national institute of health research, tehran university of medical sciencesesfahan, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمود جدی تهرانی | mahmood jeddi tehrani
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran.
امیراحمد اخوان | amirahmad akhavan
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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