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Journal of Arthropod-Borne Diseases، جلد ۱۰، شماره ۲، صفحات ۲۱۱-۲۲۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of Isotope 32P Method to Mark Culex pipiens (Diptera: Culicidae) in a Laboratory
چکیده انگلیسی مقاله Background : The aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope 32 P and determine the optimal concentration of it. Methods : An isotonic physiological saline solution, containing different concentration of radioactive isotope 32 P-labeled disodium phosphate (Na 2 H 32 PO4) was injected into rabbits via the jugular vein in the laboratory. Emerged Cx. pipiens were marked after feeding on rabbit. At the same time, the labeled conditions of emerged Cx. pipiens were also measured by placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside. Results : According to the label condition of Cx. pipiens after taking blood and the effect of different dosage Na 2 H 32 PO 4 on rabbit health, the optimal concentration of radioactive isotope was determined, that is, 0.1211 mCi/kg. By placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside, the emerged mosquitoes were also labeled. Therefore, feeding mosquitoes on the animal injected with radioactive Na 2 H 32 PO 4 was more prac­tical for detecting and tracing mosquitoes. Conclusion: The method was less time-consuming, more sensitive and safer. This marking method will facilitate post-bloodmeal studies of mosquitoes and other blood-sucking insects.
کلیدواژه‌های انگلیسی مقاله Radioactive isotope,Mark,Culex pipiens,Rabbit
نویسندگان مقاله chongxing زانگ | chongxing zhang
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china


guihong shi | guihong shi
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china collaborative innovation center for the origin and control of emerging infectious diseases, taishan medical university, taian, shandong 271000, china


yuqiang zhao | yuqiang zhao
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china


dongmei یان | dongmei yan
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china


huaiju li | huaiju li
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china


hongmei liu | hongmei liu
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china


itsanun wiwatanaratanabutr | itsanun wiwatanaratanabutr
department of plant production technology, faculty of agricultural technology, king mongkut amp;amp; 039;s institute of technology ladkrabang, bangkok 10520, thailand


maoqing gong | maoqing gong
shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/151
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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