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Journal of Arthropod-Borne Diseases، جلد ۱۰، شماره ۴، صفحات ۴۵۴-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Discrimination of Paederus fuscipes and Paederus littoralis by mtDNA-COI PCR-RFLP
چکیده انگلیسی مقاله Background: Linear dermatitis is endemic in Iran where most cases occur in the Caspian Sea coast and Fars prov­ince. The disease is caused by beetles of the genus Paederus which are active from early spring to beginning of au­tumn although its incidence rises from May to August. The classic taxonomy of Paederus spp. is based on the male genitalia that is very complex and needs expertise. In this study, we report a DNA-based method to discriminate Paederus fuscipes and Paederus littoralis (=syn: P. lenkoranus , P. ilsae ). Methods : Type specimens were collected from north and south of Iran. Molecular typing of the species was per­formed using restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-am­plified fragments of mtDNA-COI. Results: Sequence analyses of the data obtained in this study showed significant DNA polymorphisms. There were 89 substitutions between COI sequences of the two species. The mtDNA-COI fragment comprises several useful species-specific restriction sites comprising Hae III that could result in distinctively different species-specific PCR–RFLP profiles. The Hae III enzyme cuts the 872 bp PCR amplicon of P. littoralis into 737 and 100 bp and two small nonvisible bands whereas it does not cut P. fuscipes amplicon into fragments. Conclusion: This study demonstrates that molecular typing is useful method and allows one to differentiate between two species and is recommended for discrimination of other Paederus species, which morphologically are indistin­guishable or very difficult to be distinguished.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله سحر بذرافکن | sahar bazrafkan
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسن وطن دوست | hassan vatandoost
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

عباس حیدری | abbas heydari
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسن بخشی | hassan bakhshi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سمیه پناهی مقدم | somayeh panahi moghadam
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سعیده هاشمی اقدم | saedeh hashemi aghdam
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فاطمه محترمی | fatemeh mohtarami
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

عباس رحیمی foroushan | abbas rahimi foroushan
esfahan university of medical sciences, esfahan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

sinan anlaş | sinan anlaş
celal bayar university alaşehir, vocational school, department of entomology, alaşehir, manisa, turkey


منصوره شایقی | mansoreh shayeghi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد علی عشاقی | mohammad ali oshaghi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سید محمد ابطحی | seyed mohammad abtahi
epidemiology and biostatistics, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/325
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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