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Journal of Cell and Molecular Research، جلد ۸، شماره ۲، صفحات ۸۳-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration |
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| چکیده انگلیسی مقاله |
An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. Moreover, gene expression in the transformed leaves was quantified by ELISA method. The results obtained in this investigation indicated high level of gene expression in alfalfa leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
مازیار حبیبی پیرکوهی | maziar habibi pirkoohi
سعید ملک زاده شفارودی | saeid malekzadeh shafaroudi
حسن مرعشی | hasan marashi
سعید زیبایی | saeid zibaee
افسانه محکمی | afsaneh mohkami
صبا nejatizaxeh | saba nejatizaxeh
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| نشانی اینترنتی |
http://jcmr.um.ac.ir/index.php/biology/article/view/44884 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/481/article-481-389049.pdf |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Research Articles |
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