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International Journal of Molecular and Cellular Medicine، جلد ۱۴، شماره ۳، صفحات ۸۷۲-۸۸۵
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عنوان انگلیسی Evaluation of properties of Lactobacillus delbrueckii as an antitumor agent: effects in cell cycle, apoptosis, and gene expression in HT-29 colorectal cancer cells
چکیده انگلیسی مقاله This study investigated the anti-tumor properties of Lactobacillus delbrueckii, a probiotic bacterium, against HT-29 human colorectal cancer cells.
The primary objective was to evaluate the effects of Lactobacillus delbrueckii supernatant on gene expression, cell viability, apoptosis, and cell cycle progression in HT-29 cells. In Gilan Province, Iran, a native strain of Lactobacillus delbrueckii was identified from the supernatant of local yogurt. After treating HT-29 cells with different doses of the supernatant, the MTT test was used to calculate the IC50 value 24 hours later. Annexin V/PI staining and flow cytometry were used to evaluate the induction of apoptosis, and real-time PCR was used to measure changes in gene expression. The results showed that the supernatant of Lactobacillus delbrueckii strongly and dose-dependently reduced the growth of HT-29 cells. In addition to causing apoptosis and stopping the cell cycle in the G0/G1 phase, the therapy also increased the number of Sub-G1 cells, which is a sign of cell death. According to gene expression study, anti-apoptotic genes (AKT, Bcl-2) were downregulated while pro-apoptotic genes (PTEN, p53, and Bax) were upregulated. Interestingly, the natural isolate outperformed a conventional strain in terms of anticancer efficacy. These results demonstrate Lactobacillus delbrueckii's potential as a colorectal cancer treatment.
کلیدواژه‌های انگلیسی مقاله Lactobacillus delbrueckii, anti-tumor properties, apoptosis, colorectal cancer, gene expression, cell cycle
نویسندگان مقاله | Mohammad Reza Majid Khoshkholgh Pahlaviani
Department of Microbiology, La.C, Islamic Azad University, Lahijan, Iran.


| Amir Emami
Burn & Wound Healing Research Center, Shi.C, Shiraz, Iran.


| Seyed Mohammad Mehdi Mahmoodi
Department of Microbiology, Kaz.C, Islamic Azad University, Kazerun, Iran.


| Abbas Ali Rezaeian
Department of Microbiology, Ja.C, Islamic Azad University, Jahrom, Iran.
نشانی اینترنتی http://ijmcmed.org/browse.php?a_code=A-10-8334-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cancer
نوع مقاله منتشر شده Original Article
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