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Iranian Journal of Microbiology، جلد ۱۷، شماره ۵، صفحات ۷۸۳-۷۹۰
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عنوان انگلیسی Staphylococcus epidermidis modulates EMT-related gene expression and viability in MDA-MB-231 breast cancer cells
چکیده انگلیسی مقاله Background and Objectives: Breast tissue microbiota differs between healthy and cancerous tissues, with some bacteria influencing tumor progression. Staphylococcus epidermidis, a common skin commensal found in breast tumors, may play a role in epithelial-mesenchymal transition (EMT), a key step in metastasis. This study evaluated the effects of S. epidermidis culture and cell-free supernatant (CFS) on MDA-MB-231 breast cancer cell survival and expression of EMT-related genes Snail1, fibronectin 1 (FN1), and N-cadherin (CDH2). Materials and Methods: Different concentrations of S. epidermidis cultures and their CFS were applied to MDA-MB-231 cells. Cytotoxic effects were assessed by MTT assay at 2, 4, and 24 hours post-treatment. Real-time PCR analyzed gene expression after 24 hours of exposure to non-toxic concentrations (MOI 50 and 100 for cultures; 14% for CFS). Results: Low concentrations did not affect viability, while higher doses (MOI 100 and 14% CFS) reduced viability by up to 60% and 90%, respectively, at 24 hours. MOI 50 did not significantly alter gene expression. At MOI 100, Snail1 and FN1 were significantly upregulated, but CDH2 was unchanged. Treatment with 5% and 7% CFS significantly increased all three EMT gene expressions, indicating EMT induction. Conclusion: S. epidermidis affects EMT gene expression and cell viability, indicating potential involvement in breast cancer progression.
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نویسندگان مقاله | Farzaneh Zarei Mahmoodabadi
Department of Biology, ET.C., Islamic Azad University, Tehran, Iran


| Akram Tabatabaee Bafroee
Department of Biology, ET.C., Islamic Azad University, Tehran, Iran


| Arfa Moshiri
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Laboratory of Molecular Medicine, IRCCS Instituto Giannina Gaslini, Genova, Italy


| Seyed Davar Siadat
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Department of Biochemistry, Vanderbilt University Class of 2025, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran


| Nayereh Ebrahimzadeh
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5468
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