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JCR 2016
جستجوی مقالات
سه شنبه 18 آذر 1404
پژوهشهای آسیب شناسی زیستی
، جلد ۲۳، شماره ۱، صفحات ۱۱-۱۷
عنوان فارسی
ارزیابی اثر کشندگی عصارههای الکلی و هیدروالکلی شیرینبیان بر روی اووسیستهای آیمریا تنلا در شرایط آزمایشگاهی
چکیده فارسی مقاله
کلیدواژههای فارسی مقاله
عنوان انگلیسی
Evaluation of Lethal Effect of Aqueous and Alcoholic Extracts of Glycyrrhiza glabra on the Emeria tenella Oocysts In Vitro
چکیده انگلیسی مقاله
Aims:
Coccidiosis is one of the most common infectious diseases in poultry that causes huge economic losses.
Glycyrrhiza glabra
is a medicinal plant that used traditionally. The aim of the present study was to evaluate the effect of aqueous and alcoholic extracts of this plant on the
Emeria tenella
oocysts
in vitro
.
Materials & Methods:
The unsporulated oocysts were obtained by inoculation of 14
th
day-old broiler chicks with 75,000 oocysts. To obtain sporulated oocysts, 9g of feces samples was soaked in 2% potassium bichromate and incubated at 27°C for 72h. Aqueous and alcoholic extracts of
Glycyrrhiza glabra
in concentrations of 1, 2, and 5% were prepared and oocysts were exposed to these extracts for 48 hours. Thereafter, the number of sporulated and unsporulated oocysts were counted at 1, 12, 24, and 48 hours. In order to prevent any error, the experiments were repeated three times.
Findings:
Both extracts of
Glycyrrhiza glabra
in all tested concentrations cause a significant reduction in the number of sporulated and unsporulated
Eimeria tenella
oocysts compared with control (p<0.05). The rate of inhibitory effect of extracts had a direct relationship with exposure time, and inhibition was continuously increased over time.
Conclusion:
Alcoholic extracts of
Glycyrrhiza glabra
had a better effect than aqueous extracts. 5% alcoholic extract had the best effect. However, further studies are needed to find the best dose for the most anticoccidial effects and also to show its effects on other species of Eimeria and in animal models.
کلیدواژههای انگلیسی مقاله
Glycyrrhiza glabra,Sporulated and Unsporulated Oocysts,Eimeria tenella
نویسندگان مقاله
N. Rajaei |
Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
S. Habibian Dehkordi |
Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
R. Rahimi Junqani |
Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
M. Alijani |
Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
N. Sasannejad |
Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
نشانی اینترنتی
https://mjms.modares.ac.ir/article_19365_a4173a42230b2655d0bf53878fcc2821.pdf
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