این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
پژوهش‌های آسیب‌ شناسی زیستی، جلد ۲۸، شماره ۳، صفحات ۷۵-۸۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Determination of Epstein-Barr virus DNA and Its Association in Patients with Inflammatory Bowel Diseases (Crohn's Disease and Ulcerative Colitis) Using PCR, and IgM Antibodies
چکیده انگلیسی مقاله Introduction: This study investigated the association between Epstein-Barr virus (EBV) infection and inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC).
Methods: This cross-sectional study included 90 participants in three (CD, UC, control) groups, with 30 individuals in each group. Serum samples were analyzed for EBV-IgM antibodies using immunoassay and for EBV-DNA via TaqMan-PCR. Statistical methods included one-way analysis of variances (ANOVA), independent-samples Kruskal-Wallis tests, and receiver operating characteristic (ROC) curves analysis. Odds ratios (ORs) were calculated to assess potential risk factors.
Results: IBD patients exhibited significantly lower EBV-IgM levels than controls (p < 0.05). ROC analysis revealed: CD vs. controls: AUC = 0.707 (p= 0.004), cutoff ≤4.58 (sensitivity: 83.33%; specificity: 60%); UC vs. controls: AUC = 0.646 (p= 0.042), cutoff ≤7.5 (sensitivity: 100%; specificity: 40%). EBV-DNA was detected in only 3 CD patients (5.4% male, 4.3% female). EBV-IgM served as a negative predictor for IBD, while PCR provided no additional diagnostic value over serology. Advanced age was a significant risk factor for IBD (OR: 1.0812; p< 0.0001), whereas, increased EBV-IgM serum level is protective against IBD involvement (OR: 0.7739; p= 0.002).
Conclusion: Our findings indicate that advanced age constitutes a significant risk factor for IBD. The observed reduction in EBV-IgM levels among IBD patients suggests an impaired acute-phase immune response to EBV infection. However, to establish a definitive association between EBV and IBD, we recommend conducting comprehensive epidemiological studies in Iranian CD and UC populations to better characterize EBV infection status in these patient groups.
کلیدواژه‌های انگلیسی مقاله Epstein-Barr virus,Polymerase Chain Reaction,ulcerative colitis,Crohn’s disease,immunoglobulin M
نویسندگان مقاله Mojdeh Meghdadian |
Department of Biochemistry, Islamic Azad University, Damghan Branch, Damghan, Iran

Abdorrahim Absalan |
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Iran University of Medical Sciences and Health Services, Tehran, Iran

نشانی اینترنتی https://mjms.modares.ac.ir/article_24143_0e0d5fd5ae503750a2239c4373c68a1f.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات